Abstract
The effect of the tested substances on nematicidity and nematostaticity in vitro was carried out on saprobiotic (Caenorhabditis elegans, Turbatrix aceti) and phytoparasitic nematodes (Ditylenchus destructor). Previously prepared solutions of the tested substances in dilutions: 1) 1:1000, 2) 1:100, 3) 1:10, 4) 1:1, 5) not diluted, 6) control – bottled water. A solution of these concentrations was introduced into the wells of the die 0.5 ml in 4 repetitions, then a well-mixed nematode suspension with a volume of 0.5 ml was added on average 50±10 copies of the nematodes. After 24 hours of being together, mobile and non-mobile nematodes were counted, then after 48 and 72 hours, the nematode count was repeated. At the end of the experiment, the nematodes were washed from the drug in order to determine the nematostatic effect. The nematode counting operation was repeated. The nematicidal and nematostatic efficacy of the tested substances was compared with the control variant. As a result of studies on the nematicidity and nematostaticity of plant extracts, juices, antibiotics and medicaments, it was found that the developed in vitro screening method is effective, it was found that after 24, 48, 72 hours of screening, the percentage of mortality of nematodes Caenorhabditis elegans, Ditylenchus destructor, Turbatrix aceti practically did not change. In variants without dilutions, nematode mortality ranged from 70% to 100%. As dilution proceeds, the nematicidal efficacy of the drugs decreases. Dilution of preparations 1:1000 had no effect on the viability of nematodes. In vitro screening for nematicidity of different groups of biologically active substances showed that farmayod, phytoverm, horseradish juice, garlic, ginger have nematicidal properties.
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