Study of Interleukin-10 Gene Expression in Breast carcinoma in Telangana Population

Abstract

Background: Carcinoma breast is the second most common malignancy next to carcinoma cervix in India andabroad. Hence, the expression of IL-10 m-RNA gene in tumour can be a novel prognostic indicator.Method: 30 mastectomy specimens of carcinoma Breast patients aged between 30 to 70 years were studied. 2to 5 gram of tumour tissue and equivalent sample from normal peri-tumoral breast were taken from resectedbreast immediately after surgery and preserved in RNA-later solution. IL-10 m-RNA expression was detectedand quantified by RT-PCR analysis through TaqMan chemistry, on 30 samples and 30 controls. After fixation withformalin 2 micro sections of 4-5micron thickness were taken from tumor area and corresponding paraffin blockswere prepared, mounted, on albumin coated slide for H & E staining and results were observed and recorded.Results: Histologically, 08 specimens were diagnosed as grade-I (26.5%), 16 were grade-II (53.5%) and 06 weregrade-III (20%). 10 specimens (33.5%) had reactive lymph node, and the remaining 20 (66.5%) had metastaticlymph node. Mean RQ values for IL-10 were calculated by the formula for comparative ct (cycle threshold)method. 26 neoplastic tissue specimens (86.5%) showed strong expression of IL-10 mRNA, while only 2 (6.5%)normal peritumoral surrounding tissue showed IL-10 expression. 4 (13.5%) neoplastic tissue and 28 (93.5%) peritumoraltissue showed weak expression of IL-10. All grade 3 tumours showed strong expression of IL-10.Conclusion: Present pragmatic study had evidence that, IL-10 m-RNA gene expression was significant in neoplasticbreast tissue and may have potential prognostic significance.