Abstract
AbstractA new method of measurement of the binding constant of oligonucleotides with complementary polynucleotide chains was developed using a gel filtration procedure. Data for complexing of poly U with triplets ApApA are presented and analyzed by means of a simple statistical theory of cooperative adsorption deduced by the Bethe‐Guggenheim method. The complexes are triple, they contain two polymer chains bound by oligomers. The energy of stacking is found equal to 1300 cal/mol in fair agreement with earlier data of Ts'o. The energy of binding of a single triplet to both complementary chains is equal to 14,000 cal/mol.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Published version (
Free)
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
