Abstract
Objective To study the effects of interferon-β(IFN-β) gene inducing human gliomas apoptosis and to observe whether interferon-β can induce up-regulating of Fas expression. The relationship between cell apoptosis inducing by IFN-β gene and up-regulating of Fas expression was discussed.Methods To establish the SHG44 glioma model of nude mice. Eukaryonic expression vector (pSV2IFNβ) for IFN-β packaged by liposome was injected into SHG44 glioma of nude mice;and observe the glioma growth and calculate tumor size. The effects of IFN-β gene inducing human gliomas apoptosis and Fas expression were realized by immunohistochemistry and tunel.Results The expression of IFN-β gene in the glioma nude mice were determined. The glioma growth of nude mice was inhibited. IFN-β can induce glioma cell SHG44 apoptosis. Fas expression of glioma cell is significantly higher than that of the control group.Conclusion IFN-β gene can inhibit the growth of human gliomas and induce the apoptosis of human glioma cells. The apoptosis inducing by IFN-β gene may depend on the up-regulating of Fas expression.
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