Experimental study in vivo of the inhibitory effect of SEPI7 gene therapy for U251 MG human glioblastoma xenograft in nude mice

Abstract

Objective To investigate the growth - inhibitory and anti - invasion effects of SEPT7 gene on U251 MG human glioma cells in nude mice in vivo. Methods Nude mice bearing subcutaneous U251 MG glioma xenograft tumor model was set up and treated with SEPT7 recombinant adenovirus. The general status of the mice was monitored and the tumor volume was measured every three days. After treatment for 4 weeks, all the tumors were removed and the expression of SEPT7 and other invasion - related proteins ( MMP2 ,MMP9 ,MT1 - MMP.TIMP1 .TIMP2) and integrin αvβ3 were examined. Results SEPT7 could inhibit tumor growth. The average tumor volume of SEPT7 treatment group was smaller than those of the control group and empty vector group significantly. In SEPT7 treatment group, the SEPT7 expression was up - regulated. The expression of MMP2 ,MMP9,MT1 - MMP and integrinαvβ3 was lowered, while TIMP1 and TIMP2 was activated. Conclusion SEPT7 gene therapy has inhibitory effect on the invasion and growth of human gliomas. Key words: Gliomas; SEPT7; Invasion; Gene therapy; MMPs, TIMPs, Integrin