Abstract
The aim of this study was to determine the value of cellulase activity produced by mould toward filter paperase (FP-ase) and endoglucanase (EG). Penicillium sp., Aspergillus niger and Trichoderma viride utilized as mould isolate. Cogon grass substrates pre-treated with 2% NaOH. It determined the reducing sugar. The respective cellulase activity of each isolate was measured using filter paperase (FP-ase) and endoglucanase (EG). Pre-treatment successfully damaged lignin structure. The most favourable incubation period produced in 7th days. Moreover, it increased surface area of cellulose with a cellulose level of 48.32%, hemicellulose level of 9.88% and lignin level of 1.14%. Aspergillus niger exhibit the highest cellulase activity with enzymes value of 3.97 U/ml for filter paperase (FP-ase) and 4.53 U/ml for endoglucanase (EG). Penicillium sp. reached 3.71 U/ml for filter paperase (FP-ase) and 4.11 U/ml for endoglucanase (EG) while Trichoderma viride got 3.37 U/ml for filter paperase (FP-ase) and 3.38 U/ml for endoglucanase (EG).
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