Abstract
Amylase is very essential for the conversion of starches into oligosaccharides. Alpha- amylase (1, 4-alpha-glucan-glucanohydrolases) are extracellular enzymes which hydrolyze 1, 4-Glycosidic bonds and also are endoenzymes split the substrate in the interior of the molecule. Bacillus subtilis gave maximum production of alpha amylase as compared with the other strain. The study showed that the protein content and enzyme activity are high in different samples. Soil sample was collected from sugarcane waste dumping site, Muradnagar, Ghaziabad, India. Sample were inoculated in two different media using banana peel waste as an alternative source of carbon, which is an economic and profitable due to the inherent nature of the banana waste itself, for α-amylase production by Bacillus subtilis as standard strain and by isolated culture from soil sample. The highest α-amylase enzyme production and protein content were detected in Media - II. We report that α-amylase activity was detected in the crude sample of Media - II at pH 7 and temperature 35◦C using maltose substrate. The isolated culture from soil sample shows high activity of α-amylase enzyme as compared to Bacillus subtilis standard strain. The α-amylase enzyme was used in the starch processing food, detergent, textile, paper, clinical and analytical industry. © 2015 iGlobal Research and Publishing Foundation. All rights reserved.
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