Study of Bioaccumulation, Hematological Parameters, and Antioxidant Responses of Carassius auratus gibelio Exposed to Dietary Lead and Bacillus subtilis.

Abstract

Lead (Pb) is one of the most ubiquitous and toxic elements in the aquatic environment. Bacillus subtilis (B. subtilis) is a widely used probiotic in aquaculture. The aim of this study was to explore the toxic effects on bioaccumulation, hematological parameters, and antioxidant responses of Carassius auratus gibelio (C. gibelio) exposed to dietary lead at 0, 120, and 240mg/kg and/or B. subtilis at 109cfu/g. At 15 and 30days, the fish were sampled and bioaccumulation, hematological parameters, and antioxidant responses were assessed. The result showed that B. subtilis administration can provide a significant protection against lead toxicity by reducing lead bioaccumulation in tissues, increasing the antioxidant enzymes activity, recovering δ-aminolevulinic acid dehydratase activity and optimizing the hematological parameters. Our results suggested that administration of B. subtilis (109cfu/g) has the potential to combat dietary lead toxicity in C. gibelio.