Abstract
Linear chromatographic data were obtained for the enantiomers of Tröger's base separated on microcrystalline cellulose triacetate with pure ethanol as the mobile phase. The efficiency was determined for flow-rates between 0.1 and 1.0 ml/min at temperatures of 303, 318, 333 and 348 K. The efficiency is poor (below 100 plates) at 303 K, but increases rapidly with increasing temperature. From studies of the effects of these two parameters, the band profile dispersion is attributed to flow anisotropy and adsorption-desorption kinetics. The experimental elution profiles are compared with calculated profiles as a check on the quality of the efficiency measurements.
Published Version
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