STUDY OF AZO COMPOUNDS DERIVED FROM P-CRESOL LIGAND: SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL ACTIVITY

Abstract

Azo compounds contain two aromatic rings separated by an azo (-N=N-) link. These are versatile molecules that have garnered a lot of interest in both basic and applied research. In this work, synthesis of some Azo dyes derivatives by coupling P-cresol with diazonium salts obtained from p-chloroaniline and p-methoxyaniline. The structure of the synthesized compound has been characterized by using techniques like UV-Vis Spectroscopy, and Fourier-transform infrared spectroscopy (FTIR). In addition, the antimicrobial activity of these synthesized dyes compounds was examined by using the disc diffusion method against gram negative and gram positive bacteria Staphylococcus aureus and Pseudomonas aeruginosa which have been isolated from the patients of wound infection. Further, synthesized compounds showed moderate to significant inhibitory effect at the selected concentrations against the tested microorganisms. The results of antibacterial activities demonstrated that compound p-chloroaniline had good antibacterial activity against S. aureus and P. aeruginosa at the concentration of 300 mg/ml with 8.079 mm and 6.16 mm inhibition zone, diameter respectively. This study synthesized two new compounds viz., p-chloroaniline and p-methoxyaniline, and among these two p-chloroaniline has good anti-bacterial activity against S. aureus. Therefore, this can serve as a new compound for the manufacture of ointment to treat wound infections.