Abstract
The Australia‐SH antigen has been studied by electron microscopy of diluted sera which had not been subject to any purification procedure. Australia‐SH antigen associated particles could be demonstrated in serum dilutions up to 1/10,000, with or without specific antiserum added. Different types of aggregates, depending on the relative amounts of antigen and antibody added to a sample, were observed in checkerboard titration experiments. Australia‐SH antigen was most readily recognized in samples with about equal concentrations of antiserum and antigen‐containing serum, or in samples with moderate antigen excess. For optimal results, serum dilutions of 1/100 and 1/1,000 have been found most suitable. Particles indistinguishable from those of the Australia‐SH antigen were observed in serum of an apparently healthy student selected as a negative control. This serum sample showed negative reaction patterns with antiserum to the Australia‐SH antigen both in agar gel double diffusion tests and in complement fixation tests.
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Schedule a callMore From: Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology
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