Abstract
Complement fixation reaction and agar gel double diffusion test were made using as antigens, water or phosphate buffer saline extracts of liver from mice severely infected with Tyzzer's disease. The supernatant of the extract heated at 56 C for 30 minutes was highly reactive with immune mouse serum in the complement fixation test, while an intense precipitate band was observed only with unhealed extracts in agar gel double diffusion tests. Cross reactions were observed in both tests between liver extracts from mice infected with different strains of organisms originating from different sources and showing varied degrees of virulence. Such reactions were not shown between Tyzzerimmune serum and liver extracts from uninfected or murine hepatitis-infected mice, nor were they shown between the Tyzzer-antigen and normal or anti-murine hepatitis virus serum. The complement fixation test was used to test retired breeder mouse sera for disease contamination in mouse colonies. Positive reactions were observed in test sera collected from some breeding colonies that subsequently proved to be contaminated by the cortisone provocation test in young animals.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
