Abstract
The creation and analysis of a large variety of existing methods for the evaluation of integrated antioxidant properties are quite relevant in connection with a range of biological mechanisms of the antioxidants (AO) action. In this work, the existing methods are correlated with mechanisms of antioxidant action. It is shown that the results obtained by various methods are mainly incomparable. This can be connected with the implementation of various mechanisms of antioxidant action in methods. The analysis of the literature data presented in this review indicates the difficulty of creating a universal method and the feasibility of using integrated approaches based on the use of several methods that implement and combine various mechanisms of the chemical conversion of antioxidants. This review describes methods for studying the chelating ability of antioxidants, except for methods based on electron and hydrogen atom transfer reactions, which are currently not widely covered in modern literature. With the description of each mechanism, special attention is paid to electrochemical methods, as the interaction of active oxygen metabolites of radical and non-radical nature with antioxidants has an electron/proton/donor-acceptor nature, which corresponds to the nature of electrochemical methods and suggests that they can be used to study the interaction.
Highlights
IntroductionThe topic of antioxidants (AO) research dates to the beginning of the last century, it is still relevant [1,2,3]
The topic of antioxidants (AO) research dates to the beginning of the last century, it is still relevant [1,2,3]. This is due to the great variety of biological mechanisms of antioxidant action in the human body [4,5,6,7,8] and, the lack of possibility to create a universal method of antioxidant research
The existing methods to evaluate the integrated antioxidant properties, classified by three main chemical mechanisms coupled with main pathways of the biological action of antioxidants in a body, have been considered
Summary
The topic of antioxidants (AO) research dates to the beginning of the last century, it is still relevant [1,2,3]. Many researchers have attempted to systematize the existing integral methods of studying antioxidants [9,10,11,12,13,14,15], classifying them by the method used, the nature of the analytical signal formation, trying to get as close to the conditions in vivo as possible, etc. This topic has not subsided until now, as shown by the appearance of new review works [16,17,18,19]. Many of them [24,25,26,27,28,29,30,31,32,33] can be combined into a fairly coherent systematic picture in which each method will take its place and will be able to predict
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