Study association of bPRL (Bovine Prolactin) gene and milk production trait in Indonesian Holstein dairy cattle

Abstract

This study was aimed to analyze the genetic variation of bPRL (Bovine Prolactin) gene and its association to milk production in Indonesian Friesian Holstein (IFH) dairy cattle. In total 49 DNA from whole blood were extracted and followed with PCR-RFLP to distinguish the genotype difference between individual samples. The fragment of bPRL gene was amplified using pair of primer forward 5’-GAGTCCTTATGAGCTTGATTCTT-3’ and reverse 5’-GCCTTCCAGAAGTCGTTTGTTTTC-3’, resulting in 156 bp PCR product size. DNA digestion then performed using RsaI restriction enzyme that recognizes 5’-GT↓AC-3’ sites with blunt-end termini. According to the study, two genotypes were discovered that are AA and AB, with respective frequencies of 0.84 and 0.16. Based on the genotype grouping of milk production data, AB genotype yielded 4016.63±1344 kg while AA genotype yielded 3314.32±1153 Kg of milk per lactation. However, there is no statistical (P>0,05) difference between both genotypes on the amount of milk yielded. It is concluded that bPRL gene was not associated with milk production traits in the studied Indonesian Dairy Cattle population.