Study about survivin siRNA effects of proliferation and apoptosis in human lung adenocarcinoma cell line AGZY

Abstract

Objective To investigate the effects of survivin-specific siRNA on the proliferation and apoptosis of human lung adenocarcinoma cell line AGZY,and to explore their mechanisms.Methods Survivin-specific siRNA was tranfected into AGZY cells.Four groups were included:negative control group,DharmaFECT-treated group,nonsense siRNA-treated group,survivin specific siRNA-treated group.The expression of survivin mRNA levels was determined using RT-PCR.Cell growth was studied.The apoptosis was analyzed by Hoechst 33258 staining method.The protein expression levels was determined using Western blotting analyses.Results Campared with other three groups,survivin-specific siRNA could efficiently down-regulate the survivin expression at mRNA and protein levels(F =1785.25,F =38.67,P ＜ 0.05).After transfection,the cell proliferation decreased significantly compared with other three groups(F =14999.46,P ＜ 0.05).The typical changes associated with apoptosis was observed in the transfected group.Compared with other three groups,in transfected group,the expression of caspase-3 and caspase-8 was upre gulated(F =101.60,F =782.504,P ＜ 0.05),and the expression of cyclinB1 and cyclinD1 was downregulated(F =109.674,F =313.102,P ＜ 0.05).Conclusions Survivin-specific siRNA can supresses the expression of survivin,increase apoptosis,and inhibit cells proliferation.It may have relationship with the upregulation of caspase-3 and caspase-8,and the downregulation of cyclinB1 and cyclinD 1. Key words: Human lung adenocarcinoma; Survivin; SiRNA; Apoptosis; Nude mice