Abstract
Objective: To investigate and determine the antidiarrhoeal and spasmolytic potential of fruit of Aegle marmelos is due to CCBs compounds and not tannic acid.Materials and methods: The extract of ripe and dry fruit of A. marmelos (Am.Cr) was prepared in methanol: water (70:30). The antidiarrhoeal activities of the extract, loperamide and tannic acid were studied in vivo; in castor oil induced diarrhoeal model in mice whereas spasmolytic effect was studied in vitro; in isolated mice ileum. Calcium channel blocking (CCB) activity was investigated after preincubation of mice ileum by Am. Cr or loperamide and subsequent adding of K+80 mM.Results: Am. Cr inhibited castor oil-induced diarrhoea: onset of diarrhoea, total no. of faeces, and total no. of wet faeces dose-dependently, the dose of 800 mg/mL was found statistically significant, total weight of feces and total weight of wet faeces were also inhibited. Concentration-dependent inhibition of spontaneous contraction and contractile effect of K+80 mM after pre-incubation by Am. Cr were confirmed in isolated mice ileum. The results of Am. Cr are comparable with Loperamide. Tannic acid produced neither antidiarrhoeal effect nor exhibited CCB activity, however relaxant effect was observed in isolated mice ileum.Conclusion: The antidiarrhoeal and spasmolytic effects of Am.Cr may be mediated through compounds which posses CCB effect. Tannic acid exhibited relaxant effect only which is not sufficient evidence scientifically to classify as antidiarrhoeal.Bangladesh Journal of Medical Science Vol.17(2) 2018 p.205-211
Highlights
Each and every part of Aegle marmelos
In mice ileum statistically significant relaxant effect was observed (EC500.1527 μM, 95% C.I., 0.005853 to 3.986)
At the peak of depolarization rapid influx of Ca++ takes place via voltage dependant calcium channels (VDCCs) 31.Am.Cr inhibited the spontaneous contraction of mice ileum, most probably either due to intervention with the calcium release or with the calcium influx through VDCCs
Summary
Each and every part of Aegle marmelos. L; is used in traditional medicine to treat wide variety of disorders 1. Experiments were designed to evaluate the antidiarrhoeal activity of the crude extract and tannic acid both in vivo and in vitro experimental model of castor oil induced diarrhoea in mice to determine the therapeutic potential as antidiarrhoeal agent. In vivo experiments Acute toxicity studies by oral administration Acute toxicity study of the crude extract of ripe and dry fruit of A. marmelos was performed according to the method described previously[15] with some modifications. The normal saline, used to prepare different concentrations of the crude extract, had no effect on tissue contractility in the control experiments. Result In vivo experiments Acute toxicity studies The increasing concentrations of crude extract of Aegle marmelos were administered orally in the animals of test groups. Table-1: Showing the effect of toxicity study of crude extract of ripe and dry fruit of Aegle marmelos after oral administration in Swiss albino mice
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