Studies on vitamin D and related compounds XI investigations on sterols XIII the photoisomerisation of ergosterol

Abstract

Kinetic experiments on the photoisomerisation of ergosterol were carried out using different sources of light. New methods of analysis for pre-ergocalciferol and tachysterol2 had to be developed. The quantum yield of the photoisomerisation of ergosterol with ultraviolet light of 2537 Å was found to be 0.31 at 20°. This value was obtained when ether, ethanol and petroleum ether were used as solvents. Tachysterol2 is not formed from ergosterol during irradiation, but originates exclusively from pre-ergocalciferol. Excited ergosterol falls back to its ground state (69%) or transforms to pre-ergocalciferol (26%) and to unidentified products (5%).