Abstract
(1) The degree of hydrolysis both of sweet potato starch (phosphorus content 0.018-0.022%) and white potato starch (phosphorus content 0.06-0.08%) by the various kinds of the saccharifying amylases reached to the same level around 97-98%. The liberation of phosphoric acid from sweet potato starch when treated with sacchari-fying amylases obtained from Rhizopus and Asp. niger were. almost on the same level of 45-50%, but when treated with Endomyces-Saccharifying amylase it was around 25%. The results of white potato starch were almost same to that of sweet potato starch, but the phosphate liberation by Rhizopus-Saccharifying amylase was a little higher than that of Asp. niger-saccharifying amylase.(2) The second hydrol obtained from sweet potato starch by the treatment of saccharifying amylases and had a high percentage of phosphorus content showed an almost same degree of hydrolysis with that of the first hydrol. Concerning to the liberation of phosphoric acid, Asp.niger-saccharifying amylase showed the highest (70-80%), Rhizopus-saccharifying amylase followed and Endomyces-saccharifying amylase had very low activity about a half of the other amylases to sweet potato and white potato starch.(3) It was revealed that the saccharifying ratio had a tendency to be high in parallel with the liberation ratio of phosphorus acid when forth hydrol (phos-phorus content 0.030-0.045 %) was resaccharified with saccharifying amylases. Forth hydrol obtained by Endomyces-amylase showed higher saccharifying ratio when re-saccharified with saccharifying amlases than that obtained with the other kinds of the amylases. On the contrary lower saccharifying ratio was obtained when the forth hydrol of Asp. niger-amylase was re-saccharified.(4) The conclusions of this paper were as follows : Phosphatase activity of Endomyces-saccharifying amylase was low in comparison with activities of the other two kinds of amylases and amylases which contained higher phosphatase activity was most suitable for re-saccharifying purpose.
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