Abstract
Fractionation of maltooligosaccharides and isomaltooligosaccharides was carried out by the improved carbon column chromatography using a concentration gradient system of 3% n-butanol and 10% n-propanol . Excellent resolution of the oligosaccharides series was obtained. In the case of the maltooligosaccharides series, each oligosccharide was separated with wide window up to DP 12 and the similar results were obtained for the isomaltooligosaccharides up to DP 9 . Deactivation of carbon had strong influence on the resolution of oligosaccharides .
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