Studies on the Reaction between Peptides or Proteins with N-Phosphoryl Amino Acids in Aqueous Solution

Abstract

In this article, it was found that N-phosphoryl α-amino acid could react with peptide or protein in aqueous solution to form the peptides or proteins increasing one acid amino residue. Through tandem-MS (MS/MS) and liquid chromatography-tandem mass spectrometry (LC/MS/MS), it was found that the added amino acid residue was located at the N-terminal of material peptides or proteins. Furthermore, the added amino acid residue was from the N-phosphoryl α-amino acid. In this reaction process, a possible mechanism was proposed that five-membered ring penta-coordinate phosphorous intermediate played the vital role in the formation of peptide bond.