Abstract
Planar thin-layer chromatography of heavy metals has been performed on soil layers with aqueous solutions of amino acids as mobile phases. Several amino acids at different concentrations (0.5 to 5.0%) were tested to examine their effect on the mobility of the heavy metals. Increasing the concentration of the amino acids in the mobile phase resulted in increased mobility of most of the heavy metal ions studied. Neutral amino acids were capable of promoting differential migration among the heavy metals. Important separations of heavy metal ions from their mixtures were obtained with 2% aqueous solutions of neutral amino acids (alanine, serine, proline, valine, and methionine). When aqueous solutions of neutral amino acids were used as mobile phases, better separation of the heavy metals was achieved by use of a 2% solution of proline. Separations of heavy metals achieved experimentally on soil layers are listed.
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