Abstract
Two methods for separation of soybean hypocotyl are described. Solubility data are presented for the nitrogen constituents of hypocotyl extracts. Experiments indicate that removal of phytic acid is responsible for the shift in the pH of minimum solubility of hypocotyl proteins to higher values. Electrophoretic studies indicate that the interaction of phytic acid with components of the acid-precipitated protein greatly modifies its electrophoretic behavior; the major component, when phytate-free, contains two electrophoretically distinct proteins. Ultracentrifugal studies at pH 7.6 show the presence of three resolvable fractions having s 20 values of 2 S, 5 S, and 10 S, and at pH 3.0 two resolvable fractions having s 20 values of 2 S and 6 S. A comparison of the solubility and of the electrophoretic and ultracentrifugal properties of acid-precipitated proteins of the hypocotyl and cotyledon has disclosed similar as well as different properties.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
