Studies on the nucleotide arrangement in deoxyribonucleic acids. V. Pyrimidine nucleotide clusters: isolation and characterization.

Abstract

Abstract The isolation and characterization of the pyrimidine oligonucleotide clusters occuring in DNA are described. The method is based on the fractionation of acidic hydrolysates of DNA on DEAE-cellulose and on the subsequent two-dimensional paper chromatography of these fractions. Pyrimidine oligonucleotide clusters of up to nine pyrimidine units in length, and individual pyrimidine oligonucleotides up to the pentanucleotide level, have been isolated and characterized with the use of their distinctive spectral ratios and, in many instances, their composition. The fractionation method accounts for nearly all of the original DNA phosphorus.