Abstract
Single-stranded DNA and double-stranded replicative form DNA from bacteriophage S13 suN15 were purified and degraded with formic acid-diphenylamine. The pyrimidine oligonucleotides released were separated on DEAE-cellulose according to chain, length and 11 isostichs were observed for both DNA's. Each of the isostichs was then subfractionated according to base composition. Forty-seven different non-isomeric pyrimidine oligonucleotides from S13 replicative form DNA were separated, identified and quantitated. The data obtained for the single-stranded phage DNA were subtracted from that of the double-stranded replicative form, providing the oligonucleotide catalogue of the complementary strand in the replicative form DNA and the total purine, pyrimidine catalogues for both the phage and the complementary strands. The results showed an unequal distribution of oligonucleotides between the two strands, the phage single-stranded DNA being pyrimidine rich. The longest pyrimidine oligonucleotides, undecanucleotides, were found predominantly in the complementary strand. Pyrimidine decanucleotides were completely absent from the complementary strand.
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