Abstract
Publisher Summary The genome of lymphocytic choriomeningitis virus (LCMV) comprises two single-stranded RNA segments of negative polarity, which exist as ribonucleoprotein (RNP) complexes within both purified virions and infected cells. The LCMV virion also contains varying numbers of host ribosomes, but it has been suggested that these ribosomes are not required for normal virus replication. This chapter describes experiment for the DNA cloning of LCMV (1) to evaluate the full genetic potential of LCMV, (2) to generate hybridization probes for the study of LCMV gene expression and gene regulation, (3) to examine sequence relatedness between different LCMV strains and other arenaviruses, and (4) to investigate the molecular basis of LCMV-induced disease. Bacterial colonies containing cDNA inserts were screened by colony hybridization using purified and partially hydrolyzed LCMV L or S virion RNAs as probes.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
