Abstract
During the course of investigations on the metabolism of 7, 8-diaminopelargonic acid by microorganisms, it was found that a strain of Pseudomonas effectively converted 7, 8-diaminopelargonic acid into biotin vitamers. The vitamers formed were separated by the ion exchange column chromatography, into Fraction A and Fraction B. The vitamer I was isolated from the Fraction A in powder form. The isolated vitamer I did not support the growth of Saccharomyces cereaisiae, but did support that of Bacillus subtilis, and its Rf values in several system were identical with those of authentic bisnordesthiobiotin. The vitamer II which was shown to support the growth of Saccharomyces cerevisiae and Bacillus subtilis, was isolated from the Fraction B in crystalline form. The crystals were identified as desthiobiotin by physico-chemical and biological examinations. Then, the metabolic route of 7, 8-diaminopelargonic acid which included the carboxylation and β-oxidation are proposed in this paper.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call