Abstract
A novel ruthenium(II) porphyrin complex, [Ru(phen)2MPyTPP] (phen = phenanthroline, MPyTPP = mono-(3′-Pyridine)-10,15,20-triphenyl porphyrin), has been prepared and characterized by elemental analysis, ESI-MS and NMR methods. The interaction of the complex with G-quadruplex DNA has been studied by spectroscopy, and the results have shown that [Ru(phen)2MPyTPP] can bind to G-quadruplex d(TTAGGG)4 with high affinity. In the presence of d(TTAGGG)4, the characteristic Soret and intra-ligand (IL) transitions in the UV–Vis spectrum of the complex exhibit hypochromism of 25% (Δλ = 6 nm) and 18% (Δλ = 1 nm), respectively. The intrinsic binding constant was calculated according to the decay of the Soret band as 3.02(±0.08) × 106 M−1. The positive signal of d(TTAGGG)4 in its circular dichroism spectra was clearly decreased in the presence of the complex, indicating the conformation of the G-quadruplex was severely disturbed by the ruthenium(II) complex.
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