Abstract
This study was aimed at evaluating the kinetic properties and capacities of water (GWE), 50% ethanolic (GE50) and 95% ethanolic (GE95) extracts from Graptopetalum paraguayense for the potential to inhibit angiotensin converting enzyme (ACE). The results showed that GWE, GE50 and GE95 showed potent inhibitory effects on ACE. It was found that the ACE inhibitory activities of all the tested extracts increased with the increase of their concentrations. In addition, the ACE inhibition of the tested extracts of G. paraguayense were significantly reduced after the addition of 1.5 mM ZnCl 2, suggesting the inhibitory action of the extracts may have resulted from the chelation of the ACE zinc cofactor. The inhibition kinetics, analyzed by Lineweaver–Burk plots, revealed that G. paraguayense extracts showed a mixed-type inhibition. A comparison of the 50% inhibition concentration (IC 50) and K i values showed that the ethanolic extracts, including GE50 and GE95 exhibited the more effective ACE inhibitory activity than the water extracts of G. paraguayense.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
