Abstract
A comparative study of the chromosomes from root-tip cells and from pollen tube generative cells in Allium fistulosum has been carried out by means of Giemsa C-banding techniques and fluorochromes. Culture of pollen tubes was carried out on the inside surface of a spitz-type glass centrifugation tube which contained suitable amount of liquid medium contained 0.02% colchicine. In root-tip chromosomes, when used C-banding techniques, deeply stained satellites and distal banded ends were observed and they were completely corresponded to the reduced fluorescence bands as for appearing site and size dimension. Centromeric bands, proximal ones in the gap on the shortest submetacentric chromosomes (SM4) and additional interstitial ones (of which chromosomes were SM4 and M3) were found occasionally in the C-banding patterns. Inter-plant variation of C-banding patterns was observed. No organ specificity was recognized in both the karyotype and the C- and Q-banding patterns between the chromosomes from root-tip cells and pollen tube generative cells. Several difficulties present in the banding techniques applied to the pollen tube generative nucleus were discussed.
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