Abstract
1. The biosynthesis of biotin-S35 from H2S35O4 and desthiobiotin by the washed cell suspension of biotin and sulfur-deficient Saccharomyces cerevisiae was investigated with the following two methods.2. The biotin and sulfur-deficient cell suspension was incubated with sulfate-S35 and D-glucose, and the fate of sulfate-S35 was investigated. L-Methionine-S35 was found to be produced predominantly.3. The experiments with L-methionine-S35 and desthiobiotin showed the formation of biotin-S35 in a good yield.4. Using 140μc of L-methionine-S35, 0.26μc of biotin-S35 was obtained by the biotin-avidin complex method, followed by autoradiography, bioautography and paper chromatoscanning.5. From these findings, it is concluded that 70 per cent of the radioactivity in the biotin concentrate is located in biotin-related compounds, i.e., biotin-D-sulfoxide, biotin-L-sulfoxide, biocytin and biocytin sulfoxide.
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