Abstract
The whole-cell patch-clamp technique was employed to record membrane currents from isolated horizontal cells in culture. Concentration-response relationships for currents induced by l-glutamate, kainate and quisqualate were measured. Preincubation with dopamine changed the parameters of the concentration-response curves in a particular way for each agonist. The maximum currents induced by glutamate increased by 50–100% and the EC 50-values were slightly shifted to higher values. The increase of kainate-induced maximum currents after dopamine incubation did not exceed 30% but the EC 50-values were clearly shifted to lower concentrations. Quisqualate-indnced maximum currents were not enhanced by dopamine preincubation but the EC 50-values were shifted to lower concentrations. The dopamine-dependent modulation was affected by removel of magnesium and preincubation with concanavalin A and aniracetam. The concentration-response relation and the time-course of the dopamine effect on glutamate-induced currents is described.
Published Version
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