Abstract
Before studying the effects of beta-irradiation on the activity of acid and alkaline phosphatases of albino guinea pig epidermis, it was first necessary to characterize these enzymes in normal tissue. Although there is a considerable literature on the histochemical localization of these enzymes in skin (1-14), few attempts have been made to quantitate or characterize these enzymes in whole skin or its outermost layer, the epidermis (15). Our findings in this regard, utilizing albino guinea pig and rat epidermis, are presented in the following report : METHODS 1. Removal of Epidermis and Preparation of “Extra- and Intracellular Fractions: Epidermis (200-250 mg) was peeled and scraped (16) from excised skin of male and female albino guinea pigs weighing 500-900 g. The “extracellular” fraction (epidermal wash)f was obtained prior to homogenization by suspending and centrifuging excised epidermis at 4° C in several changes of 0.15 M saline and pooling the supernatants (final volume 2-3 ml). The washed tissue, containing the intracellular or cell-bound enzymatic activity, was homogenized (10% homogenate) as previously described (17).
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call