Abstract
The experiments reported in this paper were designed to answer some questions relating to the augmenting effect of storage of sperm in the inseminated female, on the frequency of translocations in spermatozoa treated with the trifunctional alkylating agent trenimon. To see whether, upon storage, more chromosome breaks become available for interaction, sperm cells that had been treated with trenimon in the male were exposed to X-irradiation before or after 6 days storage in the female. The data of the first experiment indicated that in unstored sperm the translocation yield after treatment with both trenimon and X-rays, was lower than that expected on the basis of additivity of yields of the single treatments. The negative synergism between trenimon and X-irradiation has been confirmed in further experiments with both translocations and dominant lethals. The latter finding does not support an interpretation in terms of selective elimination of translocationsby cell death. Following storage, the translocation frequencies increase and after combination of trenimon and X-rays, yields corresponding to additivity of frequencies with single treatments are observed. To study whether changes in the maternal repair system contribute to the storage effect, trenimon-treated males were mated with aged females and the frequencies of translocations were determined. It was found that these frequencies were similar to those in young (unstored) females; this result suggests that the possibility raised above is unlikely.
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