Studies on seed transmission of “Candidatus Liberibacter solanacearum” in pepper and its impact on plant emergence

Abstract

Abstract“Candidatus Liberibacter solanacearum” (Lso), transmitted by the potato psyllid (Bactericera cockerelli), is the causal agent of potato zebra chip, but can also infect other solanaceous plants, including peppers. Studies were conducted to investigate whether Lso could be transmitted to the next generation of plants through seeds from infected pepper plants. In 2014, jalapeno pepper plants were infested with psyllids carrying a mixture of Lso A and B (AB) at the AgriLife Research Station at Bushland. The study was again conducted in 2019 and pepper plants were infested with psyllids carrying Lso B or Lso AB. In each of the studies, noninfested plants served as controls. At harvest, fruits were collected and tested for the presence of Lso using quantitative PCR. Seeds from infected fruits were then tested for Lso. Overall, the percentage of seeds that tested positive for Lso ranged from 33% to 70%. However, Lso detection in embryos ranged only from 0% to 8%. Seed samples from Lso‐positive fruits were planted in the greenhouse to determine the impact of Lso on emergence and the incidence of Lso in emerged plants. Although plant emergence differed between some of the seeds obtained from Lso‐positive and ‐negative fruits, the overall impact of Lso on plant emergence was not consistent. However, of the 182 plants that emerged from seeds collected from infected fruits, none was positive for Lso, suggesting that seeds are unlikely to serve as sources for new Lso infections and their impact on disease epidemiology is negligible.