Abstract
Natural porcine secretin was purified by high performance liquid chromatography (HPLC) in order to compare it with synthetic secretin. As a starting material we used partially purified secretin obtained by extraction from porcine duodenum followed by purification by ion-exchange chromatography and gel filtration. Preparative HPLC was performed on a reverse-phase column with a volatile eluent. The biological activity of the purified secretin was 5450 c.u./mg in the anesthetized rat, which is higher than those of other natural secretins reported so far (4000 c.u./mg), and was reasonably close to that of our synthetic secretin (5750 c.u./mg). The natural and synthetic secretins were compared by several physicochemical methods.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
