Abstract
Oral administration to rats of kernels of Macrozamia communis induced changes in liver lysosomes and mitochondria prior to the onset of periacinar necrosis. Within 3 hr of poisoning with macrozamia the rate of release of β-glycerophosphate phosphatase from light mitochondrial fractions increased. This change was more pronounced after 6 hr. A similar change was induced by carbon tetrachloride and ethionine, but not by lasiocarpine or thioacetamide. Mitochondria isolated from livers of rats 6 hr after administration of macrozamia showed inhibition of NAD-dependent enzymes and an increased rate of swelling in the absence of substrate. Secretion of triglyceride from the liver of poisoned rats was inhibited, and triglyceride accumulated in the liver. Serum non-esterified fatty acid levels rose and serum triglyceride levels fell after poisoning with macrozamia. Protein synthesis was inhibited in the liver of rats treated with macrozamia. Density gradient centrifugation of post-mitochondrial and ribosomal fractions revealed a breakdown in polysomes after poisoning. The results are discussed in relation to the pathogenesis of lesions induced in the liver by macrozamia.
Published Version
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