Abstract
Photodynamic therapy (PDT) represents a promising method for treatment of cancerous tumors. The chemical and physical properties of used photosensitizer (PS) play key roles in the treatment efficacy. In this study, a novel PS, 5,10,15,20-tetrakis((5-dipropylamino)pentyl)-chlorin (TDPC) which displayed a characteristic long wavelength absorption peak at 650 nm were synthesized. It also shows a singlet oxygen generation rate of 4.257 min-1. Generally, TDPC is localized in mitochondria and nucleus of cell. After light irradiation with 650 nm laser, it can kill many types of cell, in addition, TDPC–PDT can destroy ECA-109 tumor in nude mice and a necrotic scab was formed eventually. The expression levels of many genes which regulated cell growth and apoptosis were determined by RT-PCR following TDPC–PDT. The results showed that it either increased or decreased, among which, the expression level of TNFSF13, a member of tumor necrosis factor superfamily, increased significantly. In general, TDPC is an effective antitumor PS in vitro and in vivo and is worthy of further study as a new drug candidate. TNFSF13 will be an important molecular target for the discovery of new PSs.
Highlights
Photodynamic therapy (PDT) has emerged as a promising new approach for treating and curing cancerous tumors.[1,2] It takes e®ect through localization of photosensitizer (PS) in the target tissues prior to radiation with an appropriate wavelength.[3,4]This is an Open Access article published by World Scientic Publishing Company
We further explored the changes in gene expression that occurred in response to TDPC–PDT
TDPC can be excited at 418 nm, and its emission was monitored at wavelengths 651 and 722 nm
Summary
Photodynamic therapy (PDT) has emerged as a promising new approach for treating and curing cancerous tumors.[1,2] It takes e®ect through localization of photosensitizer (PS) in the target tissues prior to radiation with an appropriate wavelength.[3,4]. This is an Open Access article published by World Scientic Publishing Company. To enhance the treatment of cancer, new PS with absorption spectra at long wavelengths (650–800 nm) which can improve tumor selectivity and a quicker clearance are urgently needed to be developed.[5]
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