Abstract
A new amperometric glucose sensor based on the glucose oxidase immobilized on pyrolytic graphite (PG) modified with tetraammineplatinum(II) chloride (TAPtCl) and 5,10,15,20-tetrakis (4-methoxy-phenyl)-21 H,23 H-porphine cobalt(II) (TMPPCo) as well as Nafion was studied. The performances amongst the glucose sensors with or without TAPtCl or/and TMPPCo measured with oxygen present in the solution were compared. The compositions of the membranes of the glucose sensors were optimized by a new orthogonal experimental design technique–sequential level elimination method according to chemometric approaches. Our studies show that the prepared sensor with optimal membrane composition in this study gives satisfactory performance in terms of long-term stability, fast amperometric response, good detection limits and satisfactory recovery. The study provides a useful basis for developing other sensors with corresponding optimal membranes.
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