Abstract
Selected 8 kinds of naphthol-sulfonic acids were classified into following 4 groups in accordance with those chemical structures.Group 1. H-acid (8-hydroxy-naphthylamine-3, 6-disulfonic acid) and Chromotropic acid (1, 8-naphthalenediol-3, 6-disulfonic acid)Group 2. R-acid (2-naphthol-3, 6-disulfonic-acid) and G-acid (2-naphthol-6, 8-disulfonic acid)Group 3. Schaeffer acid (2-naphthol-6-sulfonic acid) and Crocein acid (2-naphthol 8-sulfonic acid)Group 4. NW acid (1-naphthol-4-sulfonic acid) and oxy-L-acid (1-naphthol-5-sulfonic acid)High-voltage paper electrophoresis was carried out for the separation of naphthol-sulfonic acids mentioned above.Sørensen buffer (10% propylene glycol sol.) was used as an electrolyte, the potential gradient was 50V/cm and separation time 30 minutes.It was revealed that it was necessary to use the most suitable pH buffer for the separation of isomers of each group.These pH buffers were as follows:Group 1. Phosphate buffer (pH 6)Group 2. Borate buffer (pH 10)Group 3. Citrate or Phosphate buffer (pH 5-7)Group 4. Borate buffer (pH 10)
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