Abstract
Various isomers are always present in commercial samples of R-, G- and Schaeffer acids.According to our experiments, high-voltage paper electrophoresis seems to provide the best separation and determination of these isomers on the micro scale.The procedure was as follows.Known quantities of samples (ca. 100g) were submitted to paper electrophoresis by using borate buffer (pH 10) as an electrolyte, in the condition of 75V/cm.As soon as 10 minutes of separation time elapsed, the separated zones were cut off wet, extracted seperately with borate buffer solution (pH 10) as rapidly as possible on the water bath, the quantities of dyes in extracted fractions were fluorophotometrically determined in comprison with standard naphthol-sulfonic acids.Commercial samples were examined according to this method. The results are shown in Table 5.
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