Abstract
Objective: In this study, antifungal substances were isolated from different soil bacteria like Clostridium species in the presence of different nitrogen sources. The isolated antifungal substances were then tested against Aspergillus and Trichoderma species to analyse their potential activity. Methodology and Results: Antifungal substances were isolated from different soil bacterial species and it was observed that only Clostridium species was suitable to isolate active antifungal substances against Aspergillus and Trichoderma species in the presence of different nitrogen sources. Peptone and yeast extract were good nitrogen sources to isolate most active antifungal substances from Clostridium species. Agar well diffusion method was used to measure the zone of inhibitions and the maximum zone of inhibition was found up to 22 mm. Conclusion: Yeast extract and Peptone were found to be good nitrogen sources for the growth of Clostridium sp. YLC and antibiotic production.
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