Antibacterial Activity of Native Bacillus thuringiensis Strains from Fernandez Canyon State Park, Mexico

Lopez De La Cruz D,Barboza-Corona Je ,Valencia-Castro Cm ,Fernando Hernández-Terán ,De La Fuente-Salcido Nm

doi:10.4172/2472-1212.1000166

Abstract

Bacillus thuringiensis is a microbial insecticide whose presence in the Fernandez Canyon State Park, a protected natural area in the north of Mexico, has not been reported. The objective of this work was to isolate Bacillus thuringiensis strains isolated from the Fernandez Canyon State Park with the capacity to synthesize antimicrobial peptides (bacteriocins). We showed the isolation and characterization of two native strains of Bacillus thuringiensis (CF13 and CF42) collected from soils of the protected area. Bacteria were identified based on its capacity to synthesize spherical crystals and by sequencing of the flagellin gene. Both strains produced bacteriocins with bactericial/bacteriolytic activity against Bacillus cereus, with molecular mass of 10 kDa and 15 kDa, susceptible to proteolytic treatment, thermotolerants and with activity to ten Gram-positive and eight Gram-negative bacteria that might affect human and animal health. The importance of this work is that it is reported for the first time the isolation and characterization of bacteriocinogenic strains of Bacillus thuringiensis native from the Fernandez Canyon State Park, a protected natural area in Mexico.

Highlights

Bacillus thuringiensis is the most important microbial bioinsecticide used worldwide, and its activity is due to the production of intracellular crystals formed mainly by Cry and Cyt proteins [1,2]
The objective of this study was to select Bacillus thuringiensis strains from the Fernandez Canyon State Park based on their capacity to produce bacteriocins, with the purpose of extending the knowledge of these metabolites synthesized by this bacterium
CF13 and CF42 were selected because they had the highest antibacterial activity

Summary

Introduction

Bacillus thuringiensis is the most important microbial bioinsecticide used worldwide, and its activity is due to the production of intracellular crystals formed mainly by Cry and Cyt proteins [1,2]. There has been reports on the isolation and characterization of Mexican Bacillus thuringiensis strains from the states of Baja California Norte, Michoacán, Nayarit and Guanajuato, México [6,7] among others, few studies have focused on the capacity of these microbes to produce bacteriocins [8]. Bacillus thuringiensis is a cosmopolitan bacterium, but to our knowledge there is not report about the isolation of this microorganism in the Fernandez Canyon State Park (“Parque Estatal Cañón de Fernandez”), a protected natural area located in norther of México between the states of Coahuila and Durango, in a region known as “Comarca Lagunera” [9]. The objective of this study was to select Bacillus thuringiensis strains from the Fernandez Canyon State Park based on their capacity to produce bacteriocins, with the purpose of extending the knowledge of these metabolites synthesized by this bacterium

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Conclusion