Abstract
Using tender leaves of Valeriana as explants, 70% ethanol and 0.1% mercury as disinfectants, the effects of different disinfection methods and photoperiod on callus induction of Valeriana were studied. The rresults show that when C3 group was used to treat explants, the pollution rate and browning rate are lower, the callus induction rate was the highest. When the photoperiod was 0h/d, the healing time was the shortest, and the callus quality was the best in the experimental group, which was more conducive to the next step. The optimal disinfection combination is to soak the explants in 70% ethanol for 30 s and then soak them in 0.1% mercuric chloride for 7 min. The optimum photoperiod is 0h/d.
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More From: IOP Conference Series: Earth and Environmental Science
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