Studies on Antioxidative and Antimicrobial Activity from Hinoki Cypress Oil

Abstract

Human body and the scalp of internal, external or when symptoms occur, which often make acne and cause inflammation, Demodex in the skin, such as parasitic skin flora Staphylococcus epidermidis, Staphylococcus aureus and Propionibacterium acnes on the wall directly by stimulating hair follicles go into the dermis, causing skin irritation leads to inflammation. In this study, using Hinoki Cypress Oil Antioxidant and antimicrobial activity of skin flora and to learn, skin toner manufacturer in the clinical trials for skin flora, and antimicrobial activity by measuring the absorbance, Salmonella typhimurium Ames test carried out by using the functions were verified. Results of this study Hinoki Cypress Oil on DPPH free radical scavenging activity of 5 μL/mL in the 7.93±0.07%, 10 μL/mL and 15.49±0.18%, 20 μL/mL and 17.57±0.23%, 40 μL/mL and 20.98±0.54%, 80 μL/mL in the 28.06±0.69% appeared to have antioxidant benefits were concentration-dependent manner. However, as a result of low levels of Hinoki Cypress Oil scavenging of SC50 did not represent a positive daejomuljilin the BHT 0.1 mg/mL of the scavenging rate of 74.89±0.64% respectively. Hinoki Cypress Oil Minimum inhibitory concentration (MIC) in S. aureus appeared as a 0.02%, S. epidermidis and P. acnes is determined by the 0.01% S. aureus was higher than the Sensibility. Minimum bactericidal concentration (MBC) of S. epidermidis were determined from the 0.03%, 0.04% did not show a survival Strains. P. acnes in the 0.04% was 99.9% almost completely inactivated S. aureus of the Minimum bactericidal concentration (MBC) that was not identified. Antimicrobial activity in verification Hinoki Cypress Oil 10% from 20% show restraint in the fungus P. acnes on the two antimicrobial S. aureus and S. epidermidis to the antimicrobial activity than the three strains appear to be a very good chance that the antimicrobial functions Hinoki Cypress Oil concentration increases, respectively. Antimicrobial for skin flora and growth inhibitory concentration of skin flora skin toner P. acnes, S. epidermidis, S. aureus appeared in order, the higher the concentration, application time is longer, showed higher antimicrobial. Using the genetic toxicological safety * Correspondence should be addressed to Dr. Kyeong-Ran Kim, Department of Beauty Science, Kwangju Women’s University, Sanjeong-dong, Gwangsan-gu Gwangju, 506-713, Korea. Ph.: (062) 950-3673, Fax: (062) 950-3797, E-mail: krkim@mail.kwu.ac.kr