Abstract
SummaryThe author tried to clarify the changes of alkaline phosphatase activity and isozyme patterns in serum, liver, bone, kidney and small intestine of rats during development and organ origins of serum alkaline phosphatase appearing along with growing.Normal 30 rats were divided into 6 groups according to age and the alkaline phosphatase activity in serum and each organ was measured respectively. Alkaline phosphatase isozymes were separated by agar gel electrophoresis and the decreasing rate of alkaline phosphatase activity was measured by heat stability test.1) Organ alkaline phosphatase activity in rats was the highest in small intestine followed by the kidney and bone, and the lowest in the liver. In the change of alkaline phosphatase activity in organs according to age, no remarkable fluctuations were noted in the small intestine and the liver. In the kidney, a transient rise was noted in group 4. In the bone, the highest value was o btained in group 1 with gradual decrease, a slight rise was noted in group 5 and the lowest value in group 6.2) Serum alkaline phosphatase activity in rats was 2 to 3 times higher than that in human. The higher activity was noted in group 1 and 5, and the lowest in group 6.3) In alkaline phosphatase isozyme patterns in organs, the liver showed 2 bands and the other one band, and these isozymes showed different electrophoretic migration rates respectivery. The alkaline phosphatase isozyme pattern of liver consisted of two bands (L‐Ap1 & L‐Ap2), and showed characteristic changes during development.4) The isozyme pattern of serum consisted of two bands (S‐Ap1 & S‐Ap2)showed unique change during development.5) Heat stability test of serum and the organ alkaline phosphatase revealed that the order of the stability was as follows; L‐Ap1 in the liver, the small intestine alkaline phosphatase, the kidney alkaline phosphatase, S‐Ap2 in serum, the bone alkaline phosphatase, S‐Ap2 in serum, and L‐Ap2 in liver.6) These findings suggested that S‐Ap1 in the serum was originated from the bone, and S‐Ap2 in the serum was the mixture of small intestinal alkaline phosphatase and L‐Ap2 of the liver. To sum up, the serum alkaline phosphatase of the rats in young age appeared to originated from bone and in the relatively old age group, other serum alkaline phosphatase originating from small intestine and or liver appeared to be added.
Published Version
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