Studies of the Structure of Insulin Fibrils by Fourier Transform Infrared (FTIR) Spectroscopy and Electron Microscopy

Abstract

Fibril formation (aggregation) of insulin was investigated in acid media by visual inspection, transmission electron microscopy (TEM), and Fourier transform infrared (FTIR) spectroscopy. Insulin fibrillated faster in hydrochloric acid than in acetic acid at elevated temperatures, whereas the fibrillation tendencies were reversed at ambient temperatures. Electron micrographs showed that bovine insulin fibrils consisted of long fibers with a diameter of 5 to 10 nm and lengths of several microns. The fibrils appeared either as helical filaments (in hydrochloric acid) or arranged laterally in bundles (in acetic acid, NaCl). Freeze–thawing cycles broke the fibrils into shorter segments. FTIR spectroscopy showed that the native secondary structure of insulin was identical in hydrochloric acid and acetic acid, whereas the secondary structure of fibrils formed in hydrochloric acid was different from that formed in acetic acid. Fibrils of bovine insulin prepared by heating or agitating an acid solution of insulin showed an increased content of β‐sheet (mostly intermolecular) and a decrease in the intensity of the α‐helix band. In hydrochloric acid, the frequencies of the β‐sheet bands depended on whether the fibrillation was induced by heating or agitation. This difference was not seen in acetic acid. Freeze–thawing cycles of the fibrils in hydrochloric acid caused an increase in the intensity of the band at 1635 cm−1 concomitant with reduction of the band at 1622 cm−1. The results showed that the structure of insulin fibrils is highly dependent on the composition of the acid media and on the treatment. © 2001 Wiley‐Liss Inc. and the American Pharmaceutical Association J Pharm Sci 90: 29–37, 2001