Abstract

Neutron activation analysis and spectrophotometric phosphorus analysis gave quantitative determinations of elements in female gametophytes and embryos from mature seeds of 11 Pinus species. High concentrations of P, K, Mg, and S and significant concentrations of Cl, Ca, Mn, Zn, and Fe were detected in both tissues. Within each species of Pinus, the total mineral nutrient concentrations in haploid female gametophyte samples were similar to total mineral nutrient concentrations within diploid embryo samples. Seed size and weight varied greatly among the 11 species of Pinus investigated. P and Mg concentrations in female gametophytes and (or) embryos were not correlated with seed weight. Interspecific seed weight was negatively correlated with female gametophyte concentrations of S, Ca, Zn, and Fe, and also negatively correlated with embryo concentrations of Mn, Zn, and Fe. Interspecific seed weight was positively correlated with embryo concentrations of K and Cl. On a per tissue basis, larger seeded species contained higher total amounts of all the measured elements than small-seeded species. Key words: Pinus, seed weight, female gametophyte, neutron activation analysis, phosphorus analysis, mineral nutrient stores.

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