Abstract
SUMMARY Investigation was made of the sensitivity to laryngotracheitis virus (LTV) of chicken embryo kidney (CEK) and chicken kidney (CK) cultures derived from White Leghorn (W. Leg.) and New Hampshire by Columbian (N.H. X Col.) crossbred chickens. W. Leg. CEK cultures were most sensitive, and N.H. X Col. CK were least; W. Leg. CK and N.H. X Col. CEK were intermediate and equal. Plaques on CEK cultures were turbid from fibroblasts, while plaques on CK cultures were clear. W. Leg. CEK and N.H. X Col. CK cultures produced equal quantities of virus when inoculated with the same quantity of virus. A 10-fold increase in inoculum increased virus production 10-fold. The amount of virus produced after serial passage of LTV in tissue cultures dropped considerably, although there was no diminution of cytopathic effect. No evidence was found that LTV-infected cells produce interferon.
Published Version
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