Abstract
The present study represents a preliminary analysis of the cytochrome b protein sequences in the salmo trutta fario, salmo trutta caspius, salmo trutta trutta and salmo salar has been determined. The cytochrome b gene consisting 1141base pairs that encoding 380 amino acids and has been five origins ORF sites from nucleotides number of 125 to 718 which placed number of 72, 40, 37, 67, 32, respectively from first to end of gene. The amino acids sequences were compared with those of other salmonids such as salmo salar, salmo trutta and salmo trutta caspius, in this regards, the sequences aligned by DNAMAN program computer, results are shown regards salmo salar, salmo trutta trutta has 380 amino acids and same homology almost 99% but regards salmo truttta caspius, has 78% homology. There were more variation from 20 first amino acids than other parts of segments, however there is not full length of cytochrome b gene in samlo trutta caspius. The sequence features of the 3 RD structure protein accessed and compared between species of salmonids, The results are shown there are same homology between structures of protein.
Highlights
The salmonid fishes are one of the best studied native in the world
In Iran Salmo trutta fario and salmo trutta caspius is widely spread in a large number of water streams from the north of Iran, they are living in South of Caspian Sea that those are endemic in Caspian Sea, these fishes for reproduction migrates to rivers connected to Sea
In this study we examined the codon site of protein coding gene of the cytochrome b to identify what role natural selection may have played in divergence of the cytochrome b encoded protein among salmonids, what changes, if any, correlate with specific structures within the cytochrome b protein in other salmonids
Summary
At present there are major studies on the phylogenetic salmonid population in Norway, Chile, Canada, Scotland (Bernatchez et al, 1992), Iran (Rezaei et al, 2011, a, b,) and elsewhere). In Iran Salmo trutta fario and salmo trutta caspius is widely spread in a large number of water streams from the north of Iran, they are living in South of Caspian Sea that those are endemic in Caspian Sea, these fishes for reproduction migrates to rivers connected to Sea. Vertebrate mtDNA is a circular genome approximately 16 kb in size in most taxa and contains 37 genes that encode 13 proteins, 22 tRNAs, and 2. Cytochrome b is important in the respiratory system of cell, has major function belong to marker genetics and studies of population in solmonids (Bernatchez et al, 1992). In related to was found 380 amino acids from length of cytochrome b in salmo trutta fario, that same number and has high homology with other salmonids reported in GeneBank system
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