Abstract
The cytochrome b gene in the salmo trutta fario has been sequenced and characterized and deposited in GeneBank, Accession Number (JN995186), the gene of cytochrome b approximately 1.2 kb. and consists of one exon from first to end of the gene, as found for all of salmonids including, salmo salar, salmo trutta caspius, salmo trutta fario that reported in GeneBank. There were the first to end of gene repetitive regions C-G that was unique for cytochrome b gene. At this study DNA extracted from muscles of salmo trutta fario, after running PCR on the gel, the PCR products purify and sequenced. The fragments aligned with BLAST Network system, the results are shown there were high homology between solmonids, the rate of homology between salmo salar 93%, salmo trutta caspius almost 95% and salmo fario (was reported in GeneBank) 93%. However, the homology of sequences were very high, but the shape of salmo trutta fario and other salmonids different, salmo trutta fario has red to purple color dots on the skin but regards salmo salar and salmo trutta caspius have grey to black dots colors on the skin.
Highlights
Salmonids constitute one of the most manipulated fish in temperate countries
We studied regard mitochondrial DNA in salmo trutta fario
There is some different the shape of salmons, salmo trutta caspius and salmo salar with salmo trutta fario but the analysis are shown homology was 93 percent, especially with salmo salar. These results innovated that salmo trutta fario in Iran is originated from salmo salar that the ancestor of salmo trutta fario had migrated from Atlantic Ocean to White Sea and to Caspian Sea, because we collected samples has been connected from Rivers to Caspian Sea
Summary
Salmonids constitute one of the most manipulated fish in temperate countries. Salmonid fishes, and specially the salmo trutta fario. Salmo trutta fario is a native salmonid from Eurasia to North Africa. Present there are studies regard biological activities on the salmo trutta fario, including, marker genetics that contains microsatellites, alozymes which along with LDH5 and FBP1 (Poteaux 1995; Guyomard 1989) and mitochondrion DNA studies (Bernatchez et al, 1992).The using of DNA techniques and molecular researches for identification and characterization of fish for increasing production in them. We studied regard mitochondrial DNA in salmo trutta fario. MtDNA targeted methods have predominated in such studies, because of the general robustness and higher cellular copy number of mtDNA compared with nDNA (Mackie et al, 199).
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